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To ovariectomy when hormones were injected subcutaneously. Arrows projecting from dose and type of hormone in boxes indicate the equivalent type of hormone that was administered sequentially. The time of day of injection is indicated. Decidualization induction and implantation of the miniosmotic pump occurred on day 5, and animals were sacrificed on day 10. E2 = 17-beta estradiol, P = progesterone
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Stered by intracardiac injection of 0.2 mL of (125)I-BSA. The animals were allowed to recover for 15 minutes and then euthanized by decapitation. The infused and non-infused uterine horn and the ventral gastrocnemius skeletal muscle were dissected free of any fat and weighed and the amount of radioactivity was determined. The uterine permeability index was calculated as the ratio of uterine tissue
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Ing Clustal Omega. Mol Syst Biol 7: 539 Small I, Mar hal-Drouard L, Masson J, Pelletier G, Cosset A, Weil JH, Dietrich A (1992) In vivo import of a normal or mutagenized heterologous transfer RNA into the mitochondria of transgenic plants: towards novel ways of influencing mitochondrial gene expression? EMBO J 11: 1291?296 Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: m
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Ell wall material from other cell types (Haughn and Chaudhury, 2005; Haughn and Western, 2012; North et al., 2014). These advantages suggest that seed coat mucilage can yield cell wall proteomes that are potentially of higher quality than cell wall proteomes derived from other tissues. Here, we describe the extraction and proteomic analysis of the mature Arabidopsis seed coat mucilage and discuss